IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/06: Difference between revisions
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* Plastic ware used throughout to prevent alteration of iron concentration | * Plastic ware used throughout to prevent alteration of iron concentration | ||
* pH paper used to test iron stock to be around pH7 | * pH paper used to test iron stock to be around pH7 | ||
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* Initial OD Reading 30min after addition of Kanamycin and right before incubation = 0.197 | |||
* Cell Density can be obtained from graph :http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Voltage/OD600_Calibration | |||
* Placed plastic tubes with cells and medium inside the plastic jar borrowed from the Path Dept | |||
* Add SDW into package containing chemical reagents and catalyst to activate it | |||
* Package produces hydrogen gas which reacts with oxygen in air in the presence of palladium catalyst | |||
* Carbon dioxide also produced to form a CO2-enriched environment, favouring anaerobic growth by E.coli | |||
* Cultures incubated at 37°C for 2 nights | |||
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Revision as of 04:46, 7 August 2008
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Getting Promoter Plasmid and Iron Assay with Fur- MutantsPromoter Plasmid
Iron Assay with Fur- Mutants
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