IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/02

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Transformation of Bacillus

  • with fresh plates from yesterday, last protocol
Time (min) 0 20 40 60 85 105 125
OD650 for IA751 0.1192 0.1330 0.1736 0.2238 0.3124 0.4523 0.5113
OD650 for IA771 0.1144 0.1216 0.1593 0.2296 0.3476 0.4302 0.5565
  • t0 (IA751) = 100min
  • t0 (IA771) = 110min
  • Transformation

- IA751 on Cm5 and Spc 50 (control DNA less plates)

- IA751 + ECE112 (1μg of DNA) on Cm5

- IA751 + ECE153 (0.6μg of DNA) on Scp50

- IA771 on Cm5 (control)

- IA771 + ECE166 on Cm5

  • Glycerol stocks (about 20 tubes of each)

Check ECE112 stock

- Double digest with XbaI and EcoRI

- Gel : problem only one band (single digest???)

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