IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/28
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Results from Bacillus transformation with glycerol stocks
Test AmyE insertion
- 20μL of lysozyme, a few colonies for each - Cycle : 15min qt 37°C, 15min at 99°C, 1min at 4°C, 1min at 99°C and 1min at 4°C - for each PCR : 12μL of cells, 1μL of cells, 1μL of primer1, 1μL of primer2, 5μL of master mix - PCR (iGEM program) - run a gel
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