IGEM:Cambridge/2008/Notebook/Bacillus

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 12: Line 12:
|align="center" style="background-color: #444444;" |
|align="center" style="background-color: #444444;" |
<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
-
[[Image:Bacillus_button.gif|300px]] <sitesearch>title=Search this project</sitesearch>
+
[[Image:Bacillus_button.gif|300px]] <sitesearch></sitesearch>
<!-- ## END search column  ## -->
<!-- ## END search column  ## -->
|-  
|-  
Line 20: Line 20:
=Testing ''B.subtilis'' promoters and RBSs=
=Testing ''B.subtilis'' promoters and RBSs=
-
We are seeking to test the expression strength and response to inducers in several promoters in ''B.subtilis''.  
+
We are seeking to test the expression strength and response to inducers in several promoters in ''B.subtilis'', in combination with different Bacillus-specific ribosome binding sites.  
=Links=
=Links=

Revision as of 10:38, 3 September 2008


Customize your entry pages

Testing B.subtilis promoters and RBSs

We are seeking to test the expression strength and response to inducers in several promoters in B.subtilis, in combination with different Bacillus-specific ribosome binding sites.

Links

B. Subtilis Transformation Protcol - Electroporation

Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005)

  • SURE: handy expression system for B. subtilis
  • What about feedback/regulation effects of subtilin on the cell

Expression and characterization of aiiA gene from Bacillus subtilis BS-1.

  • (Some strains of) Bacillus subtilis produces a gene called aiiA that degrades AHL molecules from gram-negative bacteria
  • this is biobricked

Links to Cambridge 2007 Wiki



Retrieved from "http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Bacillus"
Personal tools