IGEM:Caltech/2007

Resources

Project Description

Background Research Papers

Protocols

• DNA Analysis through Gel Electrophoresis
• Innoculating E. Coli Cells in Liquid Culture
• DNA Fragment Ligation
• Plating and Streaking Cells
• Conventional PCR and Purification
• Titering
• Transformation of Plasmids into E. coli through Electroporation
• Isolation of Plasmid DNA through Alkaline Lysis
• Site-specific Cleavage of DNA through Restriction Endonuclease Digestion
• Measuring Cell Density, Fluorescence, and DNA concentration using SpectraMax M2 Hardware with SoftMaxPro Software

Internal

News and Announcements

Project Overview

• Recombineering

• N-antiterminator

• Q-antiterminator

• Cro

• Riboregulator

Notes from Preliminary Meetings

Progress

People

Students

Each team member undertook the design and characterization of one aspect of the system: (1) Recombineering; (2) N, Q, or Cro protein assessment; and (3) Engineering a riboregulator:

• Tania Bandyopadhyay
• Pradeep Bugga
• Deborah Jiang
• Kat Pak
• Haile Xiao

Advisors

• Faculty
  • Christina Smolke
  • Richard Murray
  • Rob Phillips

• Graduate Students
  • Katie Brenner
  • Fiona Chandra
  • Chiraj Dalal
  • Mary Dunlop
  • Joe Levine
  • Josh Michener