IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/14: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*Started a seed of DH1b cells from Nielsen's lab. | *Started a seed of DH1b cells from Nielsen's lab. | ||
<u>Ethanol</u> | <u>Ethanol</u><br> | ||
* | Miniprepped PACYC plasmid | ||
Prepared competent cells of BL21 strain to transform pacyc plasmid into. | |||
*Grew cells to O.D. of 3.1, followed procedure outlined by Nielsen Lab Procedures. | |||
Latest revision as of 00:10, 27 September 2017
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8/14Competent cells showed no growth, so new cells need to be prepared.
Ethanol Miniprepped PACYC plasmid Prepared competent cells of BL21 strain to transform pacyc plasmid into.
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