IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/13: Difference between revisions
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(Autocreate 2014/08/13 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
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==Entry title== | ==Entry title== | ||
<u>Ligation</u> | |||
TesA+RBS insert with LacI+backbone | |||
{| {{table}} border="1" class="wikitable" style="text-align:center;" | |||
|+<u>Ligation reaction</u> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Ligation (μL) | |||
| bgcolor=#cfcfcf | Control (μL) | |||
|- | |||
|| TesA+RBS || 2.0 || 0.0 | |||
|- | |||
|| LacI || 2.0 || 2.0 | |||
|- | |||
|| Ligation Buffer || 1.0 || 1.0 | |||
|- | |||
|| Ligase || 1.0 || 1.0 | |||
|- | |||
|| H<sub>2</sub>O || 4.0 || 6.0 | |||
|- | |||
Revision as of 11:30, 13 August 2014
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Entry titleLigation TesA+RBS insert with LacI+backbone
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