IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/11: Difference between revisions

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(Autocreate 2014/08/11 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
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==Entry title==
==LacI + RBA-TesA ligation==
* Insert content here...
* Antarctic phosphotase treatment of LacI sample


*Gel Extraction of RBS-TesA part: Sample was split evenly between 2 wells. Each well was collected separately and run through the standard procedure of the extraction kit (Zippy).


*DNA Concentrations
*LacI: 22.13 ng/μL
*RBS-TesA: 17.985 ng/μL
*ACC plasmid: 54.2 ng/μL
*Ligation reaction
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Revision as of 15:14, 11 August 2014

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LacI + RBA-TesA ligation

  • Antarctic phosphotase treatment of LacI sample
  • Gel Extraction of RBS-TesA part: Sample was split evenly between 2 wells. Each well was collected separately and run through the standard procedure of the extraction kit (Zippy).
  • DNA Concentrations
  • LacI: 22.13 ng/μL
  • RBS-TesA: 17.985 ng/μL
  • ACC plasmid: 54.2 ng/μL
  • Ligation reaction