IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08

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  • Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
TesA+RBS insert with LacI+backbone
  • Clean and concentrate LacI
  • Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.
Reagent RBS-TesA (μL) LacI (μL)
DNA 15.0 15.0
10X fast Digest Buffer 2.0 2.0
PstI 1.0 1.0
XbaI 1.0 .0
SpeI 0.0 1.0
H2O 1.0 1.0
37°C for 1 hour
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