IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08: Difference between revisions
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==Entry title== | ==Entry title== | ||
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P. | * Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P. | ||
: TesA+RBS insert with LacI+backbone | |||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | RBS-TesA (μL) | |||
| bgcolor=#cfcfcf | LacI (μL) | |||
|- | |||
|| DNA || 15.0|| 15.0 | |||
|- | |||
|| 10X fast Digest Buffer|| 2.0 || 2.0 | |||
|- | |||
|| PstI || 1.0 || 1.0 | |||
|- | |||
|| XbaI|| 1.0 || .0 | |||
|- | |||
|| SpeI || 0.0 || 1.0 | |||
|- | |||
|| H<sub>2</sub>O || 1.0 || 1.0 | |||
|- | |||
|| 37°C for 1 hour | |||
|- | |||
* Clean and concentrate LacI | * Clean and concentrate LacI | ||
* Run RBS-TesA on a 1% agarose gel. Gel extract on Monday. | * Run RBS-TesA on a 1% agarose gel. Gel extract on Monday. |
Revision as of 11:40, 8 August 2014
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