IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08

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Current revision (14:40, 8 August 2014) (view source)
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==Entry title==
==Entry title==
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
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{| {{table}} border="1" align=center <!-- Digestion table -->
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|- valign="top"
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| bgcolor=#cfcfcf | Reagent
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| bgcolor=#cfcfcf | RBS-TesA (μL)
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| bgcolor=#cfcfcf | LacI (μL)
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|-
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|| DNA || 15.0|| 15.0
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|-
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|| 10X fast Digest Buffer|| 2.0  || 2.0
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|-
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|| PstI || 1.0  || 1.0
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|-
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|| XbaI|| 1.0 || .0
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|-
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|| SpeI || 0.0 || 1.0
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|-
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|| H<sub>2</sub>O || 1.0 || 1.0
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|-
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|| 37°C for 1 hour
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|-
* Clean and concentrate LacI
* Clean and concentrate LacI
* Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.
* Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.

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Entry title

  • Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P.
  • Clean and concentrate LacI
  • Run RBS-TesA on a 1% agarose gel. Gel extract on Monday.
Reagent RBS-TesA (μL) LacI (μL)
DNA 15.0 15.0
10X fast Digest Buffer 2.0 2.0
PstI 1.0 1.0
XbaI 1.0 .0
SpeI 0.0 1.0
H2O 1.0 1.0
37°C for 1 hour
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