IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Entry title== | ==Entry title== | ||
* Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P. | * Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P. | ||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | RBS-TesA (μL) | |||
| bgcolor=#cfcfcf | LacI (μL) | |||
|- | |||
|| DNA || 15.0|| 15.0 | |||
|- | |||
|| 10X fast Digest Buffer|| 2.0 || 2.0 | |||
|- | |||
|| PstI || 1.0 || 1.0 | |||
|- | |||
|| XbaI|| 1.0 || .0 | |||
|- | |||
|| SpeI || 0.0 || 1.0 | |||
|- | |||
|| H<sub>2</sub>O || 1.0 || 1.0 | |||
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|| 37°C for 1 hour | |||
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* Clean and concentrate LacI | * Clean and concentrate LacI | ||
* Run RBS-TesA on a 1% agarose gel. Gel extract on Monday. | * Run RBS-TesA on a 1% agarose gel. Gel extract on Monday. |
Latest revision as of 00:10, 27 September 2017
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