IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/06

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*Obtained acc plasmid (chloro resistance). Started plate and liquid colonies.
*Obtained acc plasmid (chloro resistance). Started plate and liquid colonies.
 +
 +
ETHANOL:
 +
 +
*At 8:30 AM, O.D. of cells was approx. 1.2
 +
**Resuspended in 30 ml PBS, adjusted O.D. to match at 1.0, resuspended in PBS again
 +
**Began resting cell assay (9:15 AM):
 +
:::-Flask 1: 1 g/L pyruvate (.03 g)
 +
:::-Flask 2: 3 g/L pyruvate (.09 g)+ 1 g/L glucose (.03 g)
 +
:::-Flask 3: 3 g/L pyruvate (.09 g)(no glucose)
 +
**Flasks were kept in 32°C shaker/incubator with 1 ml samples taken for Hrs. 1,2,3,6, and 8. (24 Hr. sample will be taken tomorrow)
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Revision as of 22:03, 6 August 2014

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  • Checked plates from 8/5
  • Only + control on chloro and RBS-TesA on cholro had growth.
  • Obtained acc plasmid (chloro resistance). Started plate and liquid colonies.

ETHANOL:

  • At 8:30 AM, O.D. of cells was approx. 1.2
    • Resuspended in 30 ml PBS, adjusted O.D. to match at 1.0, resuspended in PBS again
    • Began resting cell assay (9:15 AM):
-Flask 1: 1 g/L pyruvate (.03 g)
-Flask 2: 3 g/L pyruvate (.09 g)+ 1 g/L glucose (.03 g)
-Flask 3: 3 g/L pyruvate (.09 g)(no glucose)
    • Flasks were kept in 32°C shaker/incubator with 1 ml samples taken for Hrs. 1,2,3,6, and 8. (24 Hr. sample will be taken tomorrow)
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