IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/05

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Current revision (22:33, 6 August 2014) (view source)
(7/5)
 
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==7/5 ==
==7/5 ==
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
* Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.
-
ETHANOL:
+
<u>Ethanol:</u>
*Started shake flasks of seeds started yesterday.
*Started shake flasks of seeds started yesterday.
**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
**Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol

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7/5

  • Did transformation of the ligations from yesterday of LacI with RBS-Tes with both negative and positive control.

Ethanol:

  • Started shake flasks of seeds started yesterday.
    • Took 500 μL cells, added to new 50 ml LB+ 50 μL chloramphenicol
    • Waited till O.D. reached .7, added 25 μL IPTG (.5 mM) (about 2:00 PM)
    • Let grow overnight


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