IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/04: Difference between revisions
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|| 37°C for 1 hour | || 37°C for 1 hour | ||
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*Both samples used in the Clean and Concentrate kit | *Both samples used in the Clean and Concentrate kit | ||
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|| 37°C for 1 hour then 65°C for 5 min | || 37°C for 1 hour then 65°C for 5 min | ||
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*The RBS-TesA and LacI DNA samples were quantified: | *The RBS-TesA and LacI DNA samples were quantified: | ||
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|| 16°C overnight | || 16°C overnight | ||
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<u>Ethanol:</u> | |||
*Started seeds of 3 seperate colonies of ethanol plasmid | |||
**50 ml LB w/ Chloramphenicol + picked colony | |||
**Cells didn't grow fast enough to add IPTG to start protein growth, so cells were left overnight to grow more | |||
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Revision as of 20:33, 6 August 2014
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Preparing another RBS-TesA and LacI Ligation
Ethanol:
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