IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/29: Difference between revisions
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Lanes+Length:</u><br> 1. 1 kb Ladder<br>3. | | rowspan="7" | <u>Lanes+Length:</u><br> 1. 1 kb Ladder<br>3. LacI = 2000<br> 5. TesA+RBS = 650<br> | ||
| rowspan="7" | [[image:7-29-14.JPG|400px]] | | rowspan="7" | [[image:7-29-14.JPG|400px]] | ||
|- | |- | ||
|| DNA(plasmid) || | || DNA (LacI plasmid) || 10.0 μL | ||
|- | |- | ||
|| 10X buffer || | || 10X buffer || 2.0 | ||
|- | |- | ||
|| | || PstI || 1.0 | ||
|- | |- | ||
|| | || SpeI || 1.0 | ||
|- | |- | ||
|| dH<sub>2</sub>O || | || dH<sub>2</sub>O || 6.0 | ||
|- | |- | ||
|| || | || || 20.0 μL --> 37°C/ ~1 Hr. | ||
|} | |} | ||
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Revision as of 14:40, 29 July 2014
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Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.
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