IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/29

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  • Miniprepped the lacI and the TesA+ RBS. There was a lot of cells with both.
  • Did gel of both, and those did not turn out well. The LacI was streaked due to contamination and degradation and the the TesA + RBS was too close to the well so there was probably error in either the miniprep or the digestions of them. Tes

Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.


Reagent Volume Lanes+Length:
1. 1 kb Ladder
3. TesA+RBS = 650
5. TesA+RBS = 650
7. TesA+RBS = 650
9. ETOH= 3650(ETOH backbone=2000)
DNA(plasmid) 3.0 μL
10X buffer 1.0
PSTI 1.0
EcoRI 1.0
dH2O 4.0
  10.0 μL --> 37°C/ ~35 min.
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