IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Tuesday 7/29==
* Miniprepped the lacI and the TesA+ RBS. There was a lot of cells with both.  
* Miniprepped the lacI and the TesA+ RBS. There was a lot of cells with both.  
* Did gel of both, and those did not turn out well. The LacI was streaked due to contamination and degradation and the the TesA + RBS was too close to the well so there was probably error in either the miniprep or the digestions of them. Tes
* Did gel of both, and those did not turn out well. The LacI was streaked due to contamination and degradation and the the TesA + RBS was too close to the well so there was probably error in either the miniprep or the digestions of them. TesA+ RBS was digested at the x and p sites while the lacI was digested at the s and p sites.
Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.  
Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.  


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| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | Reagent
| bgcolor=#cfcfcf | Volume  
| bgcolor=#cfcfcf | Volume  
| rowspan="7" | <u>Lanes+Length:</u><br> 1. 1 kb Ladder<br>3. TesA+RBS = 650<br> 5. TesA+RBS = 650<br> 7. TesA+RBS = 650<br> 9. ETOH= 3650(ETOH backbone=2000)<br>
| rowspan="7" | <u>Lanes+Length:</u><br> 1. 1 kb Ladder<br>3. LacI = 2000<br> 5. TesA+RBS = 650<br>
| rowspan="7" | [[image:7-29-14.JPG|400px]]
| rowspan="7" | [[image:7-29-14.JPG|400px]]
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|| DNA(plasmid) || 3.0 μL
|| DNA (LacI plasmid) || 10.0 μL
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|| 10X buffer || 1.0
|| 10X buffer || 2.0
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|| PSTI || 1.0
|| PstI || 1.0
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|| EcoRI || 1.0
|| SpeI || 1.0
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|| dH<sub>2</sub>O || 4.0
|| dH<sub>2</sub>O || 6.0
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|| &nbsp; || 10.0 μL --> 37°C/ ~35 min.
|| &nbsp; || 20.0 μL --> 37°C/ ~1 Hr.
|}
|}
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Latest revision as of 00:09, 27 September 2017

Project name Main project page
Previous entry      Next entry

Tuesday 7/29

  • Miniprepped the lacI and the TesA+ RBS. There was a lot of cells with both.
  • Did gel of both, and those did not turn out well. The LacI was streaked due to contamination and degradation and the the TesA + RBS was too close to the well so there was probably error in either the miniprep or the digestions of them. TesA+ RBS was digested at the x and p sites while the lacI was digested at the s and p sites.

Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.


Reagent Volume Lanes+Length:
1. 1 kb Ladder
3. LacI = 2000
5. TesA+RBS = 650
DNA (LacI plasmid) 10.0 μL
10X buffer 2.0
PstI 1.0
SpeI 1.0
dH2O 6.0
  20.0 μL --> 37°C/ ~1 Hr.
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