IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/28: Difference between revisions
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==Monday 7/28== | ==Monday 7/28== | ||
*Started a seed of the seed of colony #3 from plate #4. | *Started a seed of the seed of colony #3 from ligation plate #4. | ||
*Started a seed of the LacI promotor part (R0011) from Kylie | |||
Set up a digestion of RBS+TesA to be run on a gel tomorrow | |||
{| {{table}} border="1" align=center <!-- Digestion table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent (μL) | |||
| bgcolor=#cfcfcf | Volume (μL) | |||
|- | |||
|| DNA(RBS+TesA plasmid) || 10.0 μL | |||
|- | |||
|| 10X fast digest buffer || 2.0 | |||
|- | |||
|| dH<sub>2</sub>O || 6.0 | |||
|- | |||
|| XbaI|| 1.0 | |||
|- | |||
|| PstI|| 1.0 | |||
|- | |||
|| || 20.0 μL --> 37°C/ ~overnight | |||
|} | |||
Revision as of 14:42, 29 July 2014
 Project name
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Monday 7/28
Set up a digestion of RBS+TesA to be run on a gel tomorrow
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