IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Monday 7/28==
==Monday 7/28==
*Started a seed of the seed of colony #3 from plate #4.  
*Started a seed of the seed of colony #3 from ligation plate #4.  
*Started a seed of the LacI promotor part (R0011) from Kylie


Set up a digestion of RBS+TesA to be run on a gel tomorrow
Set up a digestion of RBS+TesA to be run on a gel tomorrow
{| {{table}} border="1" align=center <!-- Digestion table -->
{| {{table}} border="1" align=center <!-- Digestion table -->
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Latest revision as of 00:08, 27 September 2017

Project name Main project page
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Monday 7/28

  • Started a seed of the seed of colony #3 from ligation plate #4.
  • Started a seed of the LacI promotor part (R0011) from Kylie

Set up a digestion of RBS+TesA to be run on a gel tomorrow


Reagent (μL) Volume (μL)
DNA(RBS+TesA plasmid) 10.0 μL
10X fast digest buffer 2.0
dH2O 6.0
XbaI 1.0
PstI 1.0
  20.0 μL --> 37°C/ ~overnight