IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/24: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/07/24 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Successful Ligation== | ||
* | |||
*Checked seeds from yesterday, all grew enough for miniprepping. | |||
*Ran a digest of the three colonies picked of the ligation, as well as the ethanol plasmid, to ensure that iGEM sent us the right part, and that the ligation was successful. | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Lanes+Length:</u><br> 1. 1 kb Ladder<br>3. TesA+RBS = 650<br> 5. TesA+RBS = 650<br> 7. TesA+RBS = 650<br> 9. ETOH= 3650(ETOH backbone=2000)<br> | |||
| rowspan="7" | [[image:24.jpg|400px]] | |||
|- | |||
|| DNA(plasmid) || 3.0 μL | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| PSTI || 1.0 | |||
|- | |||
|| EcoRI || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 4.0 | |||
|- | |||
|| || 10.0 μL --> 37°C/ ~35 min. | |||
|} | |||
Revision as of 13:45, 24 July 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||
Successful Ligation
| |||||||||||||||||
