IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/23: Difference between revisions
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(Autocreate 2014/07/23 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
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== | ==Cell Picking== | ||
* | * From the transformations of the previous day, the positive control (Tet) and one plate (Plate 4 with 6μL) had growth on them | ||
* Plate 1: 1 μl of Tet with DNA (positive control) GROWTH | |||
* Plate 2: 1μl of ligation mixture (tes and rbs) NO GROWTH | |||
* Plate 3: 3 μl of ligation mixture NO GROWTH | |||
* Plate 4: 6 μl of ligation mixture 3 COLONIES | |||
* Plate 5: 1 μl RBS Backbone (negative control) NO GROWTH | |||
* Plate 6: 3 μl RBS backbone NO GROWTH | |||
* Plate 7: 6 μl RBS Backbone NO GROWTH | |||
* Warmed up lb in shaker and picked 3 colonies from plate 4 to be put for growth at 10:45 am. Each tube had 5mL of LB and a colony | |||
Mini prepped one of the EtOH tubes that was in the shaker that seemed to have more growth than the other two. Labelled it EtOH 1 | |||
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Revision as of 11:25, 23 July 2014
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Cell Picking
Mini prepped one of the EtOH tubes that was in the shaker that seemed to have more growth than the other two. Labelled it EtOH 1 | |
