IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==== | ==Cell Picking== | ||
* From the transformations of the previous day, the positive control (Tet) and one plate (Plate 4 with 6μL) had growth on them | * From the transformations of the previous day, the positive control (Tet) and one plate (Plate 4 with 6μL) had growth on them | ||
* Plate 1: 1 μl of Tet with DNA (positive control) GROWTH | * Plate 1: 1 μl of Tet with DNA (positive control) GROWTH | ||
* Plate 2: 1μl of ligation mixture NO GROWTH | * Plate 2: 1μl of ligation mixture (tes and rbs) NO GROWTH | ||
* Plate 3: 3 μl of ligation mixture NO GROWTH | * Plate 3: 3 μl of ligation mixture NO GROWTH | ||
* Plate 4: 6 μl of ligation mixture 3 COLONIES | * Plate 4: 6 μl of ligation mixture 3 COLONIES | ||
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* Plate 6: 3 μl RBS backbone NO GROWTH | * Plate 6: 3 μl RBS backbone NO GROWTH | ||
* Plate 7: 6 μl RBS Backbone NO GROWTH | * Plate 7: 6 μl RBS Backbone NO GROWTH | ||
* Warmed up lb in shaker and picked 3 colonies from plate 4 to be put for growth | * Warmed up lb in shaker and picked 3 colonies from plate 4 to be put for growth at 10:45 am. Each tube had 5mL of LB and a colony | ||
Mini prepped one of the EtOH tubes that was in the shaker that seemed to have more growth than the other two. Labelled it EtOH 1 | |||
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Latest revision as of 00:07, 27 September 2017
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Cell Picking
Mini prepped one of the EtOH tubes that was in the shaker that seemed to have more growth than the other two. Labelled it EtOH 1 | |
