IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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* Started seeds of ethanol
* Started seeds of ethanol
* Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
* Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
Plate 1: 1 μl of Tet with DNA (positive control)
* Plate 1: 1 μl of Tet with DNA (positive control)
Plate 2: 1μl of ligation mixture
* Plate 2: 1μl of ligation mixture
Plate 3: 3 μl of ligation mixture
* Plate 3: 3 μl of ligation mixture
Plate 4: 6 μl of ligation mixture  
* Plate 4: 6 μl of ligation mixture  
Plate 5: 1 μl RBS Backbone (negative control)
* Plate 5: 1 μl RBS Backbone (negative control)
Plate 6:  3 μl RBS backbone
* Plate 6:  3 μl RBS backbone
Plate 7: 6 μl RBS Backbone  
* Plate 7: 6 μl RBS Backbone  





Latest revision as of 00:07, 27 September 2017

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  • Started seeds of ethanol
  • Did transformations of ligations (2μl of Tes with 1μl of RBS) with DH10B 50μl
  • Plate 1: 1 μl of Tet with DNA (positive control)
  • Plate 2: 1μl of ligation mixture
  • Plate 3: 3 μl of ligation mixture
  • Plate 4: 6 μl of ligation mixture
  • Plate 5: 1 μl RBS Backbone (negative control)
  • Plate 6: 3 μl RBS backbone
  • Plate 7: 6 μl RBS Backbone