IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/21

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Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 2.0 μL TesA
10X buffer 1.0
dH2O 5.0
Ligaze 1.0
  10.0 μL --> 16°C/ ~overnight
Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 0 μL TesA
10X buffer 1.0
dH2O 7.0
Ligaze 1.0
  10.0 μL --> 25°C/ ~1 Hr.
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