IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/21: Difference between revisions
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(Autocreate 2014/07/21 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014) |
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== | ==7/21== | ||
* | * Ligations started for TES with RBS and control. Following are the tables. | ||
{| {{table}} border="1" align=center <!-- Ligation table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent (μL) | |||
| bgcolor=#cfcfcf | Volume (μL) | |||
|- | |||
|| DNA(plasmid) || 1.0 μL RBS, 2.0 μL TesA | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 5.0 | |||
|- | |||
|| Ligaze || 1.0 | |||
|- | |||
|| || 10.0 μL --> 16°C/ ~overnight | |||
|} | |||
{| {{table}} border="1" align=center <!-- Ligation table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Reagent (μL) | |||
| bgcolor=#cfcfcf | Volume (μL) | |||
|- | |||
|| DNA(plasmid) || 1.0 μL RBS, 0 μL TesA | |||
|- | |||
|| 10X buffer || 1.0 | |||
|- | |||
|| dH<sub>2</sub>O || 7.0 | |||
|- | |||
|| Ligaze || 1.0 | |||
|- | |||
|| || 10.0 μL --> 16°C/ overnight | |||
|} | |||
* Transformations of Tet (Tube 4) on competency cells. | |||
* 50μL of cells with 1μL of Tet, 50μL with 5μL of Tet, and one with 50μL cells with 10μL of Tet. Plated these cells on chloramphenicol plates. | |||
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Revision as of 13:13, 21 July 2014
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