IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/21: Difference between revisions

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(Autocreate 2014/07/21 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==7/21==
* Insert content here...
* Ligations started for TES with RBS and control. Following are the tables.
{| {{table}} border="1" align=center <!-- Ligation table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent (μL)
| bgcolor=#cfcfcf | Volume (μL)
|-
|| DNA(plasmid) || 1.0 μL RBS, 2.0 μL TesA
|-
|| 10X buffer || 1.0
|-
|| dH<sub>2</sub>O || 5.0
|-
|| Ligaze || 1.0
|-
|| &nbsp; || 10.0 μL --> 16°C/ ~overnight
|}


{| {{table}} border="1" align=center <!-- Ligation table -->
|- valign="top"
| bgcolor=#cfcfcf | Reagent (μL)
| bgcolor=#cfcfcf | Volume (μL)
|-
|| DNA(plasmid) || 1.0 μL RBS, 0 μL TesA
|-
|| 10X buffer || 1.0
|-
|| dH<sub>2</sub>O || 7.0
|-
|| Ligaze || 1.0
|-
|| &nbsp; || 10.0 μL --> 16°C/ overnight
|}


* Transformations of Tet (Tube 4) on competency cells.
* 50μL of cells with 1μL of Tet, 50μL with 5μL of Tet, and one with 50μL cells with 10μL of Tet. Plated these cells on chloramphenicol plates.
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|}
|}

Latest revision as of 00:07, 27 September 2017

Project name Main project page
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7/21

  • Ligations started for TES with RBS and control. Following are the tables.
Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 2.0 μL TesA
10X buffer 1.0
dH2O 5.0
Ligaze 1.0
  10.0 μL --> 16°C/ ~overnight
Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 0 μL TesA
10X buffer 1.0
dH2O 7.0
Ligaze 1.0
  10.0 μL --> 16°C/ overnight
  • Transformations of Tet (Tube 4) on competency cells.
  • 50μL of cells with 1μL of Tet, 50μL with 5μL of Tet, and one with 50μL cells with 10μL of Tet. Plated these cells on chloramphenicol plates.
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