IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/17: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/07/17 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Ligation #3 Continued==
* Insert content here...
*Yesterdays streaked plates from the third ligation showed no growth, except for mCherry, which had maybe 15 very small colonies.
*The mCherry that was poured out onto a new plate yesterday had a full lawn.
**Considering this information, the remaining samples of the TesA/RBS ligation and the RBS backbone were poured onto new plates in the hopes that they too will show more growth.
**Plates were left upstairs in the 32°C incubator, as the incubator downstairs doesn't hold a constant temp, and its airflow drys out the plates prematurely.
*Seeds of the mCherry plate that grew were started.
**Three seeds were made using 5 ml LB with chlor. antibiotic, and one colony. The pipette tip was dipped into the first and second and then scraped along the bottom of the third.  




Revision as of 10:45, 17 July 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Ligation #3 Continued

  • Yesterdays streaked plates from the third ligation showed no growth, except for mCherry, which had maybe 15 very small colonies.
  • The mCherry that was poured out onto a new plate yesterday had a full lawn.
    • Considering this information, the remaining samples of the TesA/RBS ligation and the RBS backbone were poured onto new plates in the hopes that they too will show more growth.
    • Plates were left upstairs in the 32°C incubator, as the incubator downstairs doesn't hold a constant temp, and its airflow drys out the plates prematurely.
  • Seeds of the mCherry plate that grew were started.
    • Three seeds were made using 5 ml LB with chlor. antibiotic, and one colony. The pipette tip was dipped into the first and second and then scraped along the bottom of the third.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Arizona_State/2014/Notebook/asu_igem_2014/2014/07/17&oldid=802735"