IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/16: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 11: | Line 11: | ||
#Competent cells: | #Competent cells: | ||
#*Mixed 50 μL of competent cells, treated with the mCherry plasmid, with 500 μL of SOC and .5 μL of chloramphenicol, and incubated @ 32°C | #*Mixed 50 μL of competent cells, treated with the mCherry plasmid, with 500 μL of SOC and .5 μL of chloramphenicol, and incubated @ 32°C | ||
::After about 2 hours, no growth was seen. | |||
#*Poured remaining mixture of mCherry competent cells onto a new chlor. plate and incubated @ 32°C | #*Poured remaining mixture of mCherry competent cells onto a new chlor. plate and incubated @ 32°C | ||
#Ligation | #Ligation |
Revision as of 10:33, 16 July 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Ligation attempt # 3
|