IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/15: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| (One intermediate revision by the same user not shown) | |||
| Line 9: | Line 9: | ||
* Checked DNA concentrations | * Checked DNA concentrations | ||
:TesA= 11.99 ng/μL ; RBS=32.99 ng/μL | :TesA= 11.99 ng/μL ; RBS=32.99 ng/μL | ||
*When divided by the number of base pairs: [TesA]=.018 | *When divided by the number of base pairs: [TesA]=.018 and [RBS]=.016 | ||
* Ligation | * Ligation | ||
{| {{table}} border="1" align=center <!-- | {| {{table}} border="1" align=center <!-- Ligation table --> | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent (μL) | | bgcolor=#cfcfcf | Reagent (μL) | ||
| Line 26: | Line 26: | ||
|| || 10.0 μL --> 25°C/ ~1 Hr. | || || 10.0 μL --> 25°C/ ~1 Hr. | ||
|} | |} | ||
*After ligation, the mixture was heated to 65°C for 10 min. to denature the ligaze. | |||
*Transformation was then performed. | |||
:Three plates were grown overnight: RBS backbone (vector), TesA+RBS, and mCherryboth as a positive control and to replenish our stocks. | |||
* Transformation | * Transformation | ||
:Unfortunately, no growth was seen on any of the plates, including the positive control of mCherry. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 07:46, 16 July 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||
TesA/RBS Ligation Attempt 2
| |||||||||||||
