IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/15: Difference between revisions
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== | ==TesA/RBS Ligation Attempt 2== | ||
* Checked DNA concentrations | * Checked DNA concentrations | ||
:TesA= 11.99 ng/μL ; RBS=32.99 ng/μL | :TesA= 11.99 ng/μL ; RBS=32.99 ng/μL | ||
*When divided by the number of base pairs: [TesA]=.018 | *When divided by the number of base pairs: [TesA]=.018 and [RBS]=.016 | ||
* Ligation | * Ligation | ||
{| {{table}} border="1" align=center <!-- | {| {{table}} border="1" align=center <!-- Ligation table --> | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent (μL) | | bgcolor=#cfcfcf | Reagent (μL) | ||
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|| || 10.0 μL --> 25°C/ ~1 Hr. | || || 10.0 μL --> 25°C/ ~1 Hr. | ||
|} | |} | ||
*After ligation, the mixture was heated to 65°C for 10 min. to denature the ligaze. | |||
*Transformation was then performed. | |||
:Three plates were grown overnight: RBS backbone (vector), TesA+RBS, and mCherryboth as a positive control and to replenish our stocks. | |||
* Transformation | * Transformation | ||
:Unfortunately, no growth was seen on any of the plates, including the positive control of mCherry. | |||
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Revision as of 07:46, 16 July 2014
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TesA/RBS Ligation Attempt 2
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