IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/15

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==Entry title==
==Entry title==
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* Checking DNA concentration
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* Checked DNA concentrations
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:TesA= 11.99 ng/μL ; RBS=32.99 ng/μL
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*When divided by the number of base pairs: [TesA]=.018 strands/μL and [RBS]=.016 strands/μL
* Ligation
* Ligation
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{| {{table}} border="1" cellspacing="3"  align=center <!-- Digest check rxn. table -->
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|- valign="top"
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| bgcolor=#cfcfcf | Reagent (μL)
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| bgcolor=#cfcfcf | Volume (μL)
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|-
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|| DNA(plasmid) || 1.0 μL RBS, 2.0 μL TesA
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|-
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|| 10X buffer || 1.0
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|-
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|| dH<sub>2</sub>O || 5.0
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|-
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|| Ligaze || 1.0
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|-
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|| &nbsp; || 10.0 μL --> 25°C/ ~1 Hr.
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|}
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* Transformation
* Transformation

Revision as of 17:31, 15 July 2014

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Entry title

  • Checked DNA concentrations
TesA= 11.99 ng/μL ; RBS=32.99 ng/μL
  • When divided by the number of base pairs: [TesA]=.018 strands/μL and [RBS]=.016 strands/μL
  • Ligation
Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 2.0 μL TesA
10X buffer 1.0
dH2O 5.0
Ligaze 1.0
  10.0 μL --> 25°C/ ~1 Hr.


  • Transformation
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