IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 12: | Line 12: | ||
* Made LB agar plates | * Made LB agar plates | ||
1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes. | -1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes. | ||
* Ran Digestion of TesA and RBS | * Ran Digestion of TesA and RBS | ||
TesA cut at X, P and RBS cut at S, P. | -TesA cut at X, P and RBS cut at S, P. | ||
Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel. | Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel. | ||
Revision as of 11:04, 15 July 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||
Entry title
-1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes.
-TesA cut at X, P and RBS cut at S, P. Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.
| |||||||||||||||||||||||
