IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/14: Difference between revisions
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* Made LB agar plates | * Made LB agar plates | ||
1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes. | 1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes. | ||
* Ran Digestion of TesA and RBS | |||
TesA cut at X, P and RBS cut at S, P. | |||
Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.[[Media:HPTSMSMVP.JPG]] | |||
Revision as of 10:09, 15 July 2014
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1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes.
TesA cut at X, P and RBS cut at S, P. Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.Media:HPTSMSMVP.JPG
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