IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/14
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-1 L of distilled water, 35 grams of LB agar powder. Autoclave for 30 minutes.
-TesA cut at X, P and RBS cut at S, P.
Note: in order to ensure the viability of our PstI enzyme, we ran a control with some PstI from Haynes lab. Results are shown below. the PstI of Haynes lab had full digestion, while the iGEM PstI had a small amount of partial digestion, as made manifest by the three bands of the TesA in lane 2 of the gel.