IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/08
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Gel Extraction, New Digests, and Tentative Plans
Digest TesA at X and P sites and RBS at S and P and run overnight Later Gel: Run in gel and extract TesA part without backbone and RBS linear plasmid with backbone. Ligate: TesA part into RBS backbone part E|X| RBS | S| X| TESA | P| P| Then cut at E and P sites, gel extract, and ligate with K backbone. Same with MCherry Transform: Transform the plasmids into cells (mix plasmids with competent cells) Both plasmids into cell Plate Cells Pick colonies to make seeds/tubes Dr. Nielsons Suggestions: 1. Two plasmids 2. Attach primers to TesA and MC 3. Order Tes with RBS attached 4. Start ACL with Wax 5. Might not add MCherry to plasmid, instead add to chromosome to make permanent change later.
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