IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/03: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 10: | Line 10: | ||
'''TesA''' | '''TesA''' | ||
* Digest 15 μL DNA with | * Digest 15 μL DNA with 3 μL Fast Digest buffer w/ dye, 1μL Xba1, 1 μL Pst1, and 10μL of water. | ||
'''RBS''' | '''RBS''' | ||
Line 16: | Line 16: | ||
Let the reactions digest for at least 15 minutes | Let the reactions digest for at least 15 minutes | ||
Actual time: | Actual time: about 20 minutes | ||
Ran in a 1% agarose gel made with SafeWhite | |||
Result: | |||
Bands were faint | |||
''' | Repeated digest, but with the following procedure: | ||
'''TesA''' | |||
* Digest 15 μL DNA with 3 μL Fast Digest buffer, 1μL Xba1, 1 μL Pst1, 5 μL loading dye, and 5 μL of water. | |||
'''RBS''' | |||
* Digest 15 μL DNA with 3 μL Fast Digest buffer, 1μL EcoR1, 1 μL Spe1, 5 μL loading dye, and 5 μL of water. | |||
Let the reactions digest for at least 15 minutes | |||
Actual time: about 20 minutes | |||
Ran in a 1% agarose gel made with SybrSafe | |||
Result: Bands were inconclusive | |||
'''Final Digests''' | |||
Run digest overnight, then run in gel on 7/4 | |||
RBS with Spe1 and Pst1 | |||
TesA with Xba1 and Pst1 | |||
TesA with EcoR1 and Pst1 | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 15:02, 3 July 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Ligation reaction for RBS and TesA
TesA
RBS
Let the reactions digest for at least 15 minutes Actual time: about 20 minutes Ran in a 1% agarose gel made with SafeWhite Result: Bands were faint Repeated digest, but with the following procedure: TesA
RBS
Let the reactions digest for at least 15 minutes Actual time: about 20 minutes Ran in a 1% agarose gel made with SybrSafe Result: Bands were inconclusive
Run digest overnight, then run in gel on 7/4 RBS with Spe1 and Pst1 TesA with Xba1 and Pst1 TesA with EcoR1 and Pst1 |