IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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'''TesA''' | '''TesA''' | ||
* Digest 15 μL DNA with | * Digest 15 μL DNA with 3 μL Fast Digest buffer w/ dye, 1μL Xba1, 1 μL Pst1, and 10μL of water. | ||
'''RBS''' | '''RBS''' | ||
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Let the reactions digest for at least 15 minutes | Let the reactions digest for at least 15 minutes | ||
Actual time: | Actual time: about 20 minutes | ||
Ran in a 1% agarose gel made with SafeWhite | |||
Result: | |||
Bands were faint | |||
''' | Repeated digest, but with the following procedure: | ||
'''TesA''' | |||
* Digest 15 μL DNA with 3 μL Fast Digest buffer, 1μL Xba1, 1 μL Pst1, 5 μL loading dye, and 5 μL of water. | |||
'''RBS''' | |||
* Digest 15 μL DNA with 3 μL Fast Digest buffer, 1μL EcoR1, 1 μL Spe1, 5 μL loading dye, and 5 μL of water. | |||
Let the reactions digest for at least 15 minutes | |||
Actual time: about 20 minutes | |||
Ran in a 1% agarose gel made with SybrSafe | |||
Result: Bands were inconclusive | |||
'''Final Digests''' | |||
Run digest overnight, then run in gel on 7/4 | |||
RBS with Spe1 and Pst1 | |||
TesA with Xba1 and Pst1 | |||
TesA with EcoR1 and Pst1 | |||
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Latest revision as of 00:04, 27 September 2017
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Ligation reaction for RBS and TesA
TesA
RBS
Let the reactions digest for at least 15 minutes Actual time: about 20 minutes Ran in a 1% agarose gel made with SafeWhite Result: Bands were faint Repeated digest, but with the following procedure: TesA
RBS
Let the reactions digest for at least 15 minutes Actual time: about 20 minutes Ran in a 1% agarose gel made with SybrSafe Result: Bands were inconclusive
Run digest overnight, then run in gel on 7/4 RBS with Spe1 and Pst1 TesA with Xba1 and Pst1 TesA with EcoR1 and Pst1 |