IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/01: Difference between revisions
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* | * Gel Electrophoresis of Plasmids | ||
* Made TAE buffer with 1 L of distilled water and 20mL of 50X stock of TAE. Also prepared the gel with agarose and water mixture and let solidify. | |||
* Made plasmids ready by using 3μL of DNA and 3μL of loading dye. 5 tubes of this were made as well as 1 tube of ladder 3μL. | |||
* Insert ladder as well as DNA plasmids in wells and let sit for around 45-50 minutes. | |||
* This was to determine concentration of plasmids | |||
**Entry by Vallari Somayaji** | |||
Revision as of 13:07, 1 July 2014
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