IGEM:American University/2009/Notebook/Nanoparticles Experiment/One Click Setup

9/16/2015

Making Solutions:

Using the Magnetite hydrogel synthesis protocol to make diluted solutions of KNO3, KOH, Hemoglobin, and Cupric Acetate instead of FeSO4.

Cupric Acetate:

Mass (g) Measured: 1.4974

Molecular Weight g/mol: 199.65

Molar Concentration after diluted with 25mL H2O: 0.3 M

KOH:

Mass (g) Measured: 1.5396

Molecular Weight g/mol: 56.11

Molar Concentration after diluted with 25mL H2O: 1.0975 M

KNO3:

Mass (g) Measured: 2.2747

Molecular Weight g/mol: 101.1

Molar Concentration after diluted with 25mL H2O: 0.91315 M

9/23/2015

Making Hemoglobin and Stirring Solution with KNO3, KOH, Hemoglobin, and Cupric Acetate

Hemoglobin:

Mass (g) Measured: 0.0250

Molecular Weight g/mol: 64500

Molar Concentration after diluted with 25mL H2O: 1.5x10^-5

Using the Magnetite hydrogel synthesis protocol to make the 20mL solution.

-Heat water bath to 80°C

-Suspend solution in water bath so liquid is fully emerged.

-Mix solution with the IKA Eurostar 20 on 2500rpm for 1.5 hours.

Start time: 10:30am

End time: 12:00pm

Observations:

At 10 min: Solutions started changing from bright blue to dark blue.

At 20 min: Solutions is a very dark blue

At 40 min: Solution is fully black. Particles are starting to form above meniscus line.

AT 70 min: Solution continues to get darker.

-Put in centrifuge at 4000rpm, 3000BK, for 5 minutes

9/30/2015

-Two more 20 mL solutions where made. (Three total)

-Pictures were taken with microscope.

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