IGEM:American University/2009/Notebook/Advanced Experimental Chemistry: Fun Times in Beeghly/2013/04/23: Difference between revisions
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1) Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible. | 1) Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible. | ||
2) prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer. Set in the electrophoresis tray with the comb for 20+ minutes. | 2) prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer (microwaved together for 40 seconds). Set in the electrophoresis tray with the comb for 20+ minutes. | ||
Revision as of 09:44, 23 April 2013
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Welcome to Advanced Experimental Chemistry1) Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible. 2) prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer (microwaved together for 40 seconds). Set in the electrophoresis tray with the comb for 20+ minutes.
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