IGEM:American University/2009/Notebook/AU Quantum Dot Lab/2014/09/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|

Latest revision as of 00:17, 27 September 2017

iGEM Project name 1 Main project page
Next entry

Objective

  • Synthesize HgS NCs
  • Analyze the UV-vis and Fluorescence Spectra

Solution Preparation

5 mM Mercurous Nitrate

  • Mass needed: 0.0701g
  • Mass measured: 0.0705g
  • Volume: 50mL volumetric
  • Final Molarity: 0.00502 M HgNO3


After vigorous stirring the HgNO3 would not dissolve in, and therefore HgCl2 would be substituted for this trial.

5mM Mercuric Chloride

  • Mass needed: 0.0679g
  • Mass measured:0.0670g
  • Volume: 50mL volumetric
  • Final Molarity: 0.00494 M HgCl2

(15mg/mL) BSA

  • Mass needed: 0.75g
  • Mass measured: 0.751g
  • Volume: 50mL volumetric

Final Molarity: 15mg/mL

1M Sodium Hydroxide

  • Mass needed: 2g
  • Mass Measured:1.98g
  • Volume: 50mL volumetric
  • Final molarity 0.99 M NaOH

Protocol

  1. In a round bottom flask 5mL of 5mM HgNO3 (for this time HgCl2)
  2. 5mL BSA solution in MQ water and stir
  3. Correct the pH to approximately 9 using 1M NaOH
  4. Allow solution to stir overnight (12-18 hours)
  5. Add 4mL of 20mM Na2S and stir for 15 minutes

Notes

  • The mercuric chloride with a 4+ charge vs Mercurous nitrate with a 2+ charge, could explain the poor results seen in both fluorescence and UV-vis spectra.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:American_University/2009/Notebook/AU_Quantum_Dot_Lab/2014/09/08&oldid=1015308"