Hygromycin B
Hygromycin B is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis.
Cloning of the resistance gene and fusion with eukaryotic promoters has resulted in the development of vectors that permit selection for resistance to Hygromycin B in both prokaryotic and eukaryotic cells.
Mode of action
Interferes with protein synthesis.
Mechanism of resistance
Stock solution
Stability
- >1y at –20°C
- 3m at RT
- >1m at 37°C
sensitive to high concentrations of acid, but short-term exposure to dilute acids tolerated;
Usage notes
The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 mg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below.
| cell type | typical HygB concentration |
|---|---|
| mammalian cells | 200 mg/ml |
| plant cells & bacteria | 20-200 mg/ml |
| fungi | 200-1000 mg/ml |
For more information on working concentrations: [1]
External links
Wikipedia entry: Hygromycin B
Information from hygromycin.net (Invivogen)
