Hygromycin B

Hygromycin B is an antibiotic used to select prokaryotic and eukaryotic cells. It works by interfering with protein synthesis. Cloning of the resistance genes and fusion with eukaryotic promoters has resulted in vectors to select for resistance in both prokaryotic and eukaryotic cells.

Mode of action

Interferes with protein synthesis.

Mechanism of resistance

Resistance genes are kinases that inactivate Hygromycin B through phosphorylation.

Stock solution

Stock Solution Concentration: 50 mg/mL Solvent: H2O Solution Stability: 30 days Working Concentration: 100-200 µg/mL

Stability

• >1y at –20°C
• >2y at +4°C (http://www.hygromycin.com/service.htm)
• 3m at RT
• >1m at 37°C

sensitive to high concentrations of acid, but short-term exposure to dilute acids tolerated;

Usage notes

The working concentration for selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for resistant cells varies between 25 and 1000 mg/ml. You need to determine your optimum concentration experimentally. For some typical values by cell types see the table below.

typical HygB concentrations by cell type

cell type	HygB concentration
mammalian cells	200 mg/ml
plant cells & bacteria	20-200 mg/ml
fungi	200-1000 mg/ml

For more information on working concentrations: [1]

External links

Wikipedia entry: Hygromycin B

Information from hygromycin.net (Invivogen)

• mechanism of action: [2]
• resistance genes: [3]
• chemistry, stability: [4]