HughesLab:Protocols: Difference between revisions
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== Molecular Biology == | == Molecular Biology == | ||
* Basic qPCR protocol using the ABI7900HT machine and Taqman probes: [[Image: | * Basic qPCR protocol using the ABI7900HT machine and Taqman probes: [[Image:RNA_prep_using_Trizol.docx]]<br> | ||
* Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br> | * Arcturus RiboAmp RNA amplification from fly brains: [[Image:RiboAmp1.docx]]<br> | ||
* Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br> | * Basic RT reaction using ABI Archive cDNA kit: [[Image:RT_protocol.docx]]<br> | ||
* RNA prep using Trizol and Qiagen RNeasy columns: [[ | * RNA prep using Trizol and Qiagen RNeasy columns: [[File:TrizolRNAProtocol.v1.pdf]] | ||
* Protein Extract RIPA Recipe from Xiping Zhang at Esser Lab: [[File:EsserRIPABuffer.pdf]] | |||
* New cDNA reverse transcription protocol for WUSTL: [[File:cDNAprotocol.wustl.pdf]] | |||
* Human Muscle RNA Extraction from L.R. [[File:RNA extraction from human muscle.pdf]] | |||
<br> | |||
== Cell Culture == | |||
*C2C12 Harvest for Protein Lysate: [[File:Cultured_C2C12s_to_Protein_Lysate.pdf]] | |||
</br> | |||
== Protein Biology == | |||
* Tentative Western Protocol: [[File:WesternProtocolHughes31July2019.pdf]] | |||
</br> | |||
== Mouse Colony Management, Genotyping, etc. == | |||
* Mouse tail digestion protocol for genotyping: [[File:MouseTailingProtocol.pdf]] | |||
* PCR and Genotyping Protocol: [[File:HughesGenotypingProtocol.pdf]] | |||
* EtBr Gel Imaging Settings: [[File:EtBrGelImagingSettings.pdf]] | |||
<br> | <br> | ||
== Next-generation sequencing == | == Next-generation sequencing == | ||
* Pilot protocol for PCR amplifying fly RNA editing sites: [[Image:editingseq_pilot.docx]]<br> | * Pilot protocol for PCR amplifying fly RNA editing sites: [[Image:editingseq_pilot.docx]] | ||
* miRNA TruSeq Library Prep: [[Image:miRNA library prep protocol_Illumina.pdf]]<br> | |||
<br> | <br> | ||
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* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]] | * ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]] | ||
* Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]] | * Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]] | ||
* Fly food protocol: [[Image:Fly_food.docx]] | |||
<br> | <br> | ||
== Analytics == | == Analytics == | ||
* Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]] | * Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]] | ||
* RUM (RNA-Seq Unified Mapper) guide: [[Image:RUM guide.pdf]] | |||
* Basic linux commands: [[Image:linux commands.pdf]] | |||
* Willie's Aligning Sheet (26 March 2019): [[Image:ShellBashLinuxAligningSTAR.tips.pdf]] | |||
<br> | |||
<br> | <br> | ||
---- | ---- | ||
[[Image: | [[Image:WashU_Logo.png|left|150px]] | ||
<p style="font-size: | <p style="font-size: 170%;"> | ||
Hughes Lab<br> | Hughes Lab<br> | ||
[ | [https://pulmonary.wustl.edu/ Pulmonary and Critical Care Medicine]<br> | ||
University of | Washington University School of Medicine | ||
Latest revision as of 08:35, 3 January 2020
Home Lab Members Research JTK_Cycle MuscleDB Publications Protocols Academic Analytics Open Positions Contact
Molecular Biology
- Basic qPCR protocol using the ABI7900HT machine and Taqman probes: File:RNA prep using Trizol.docx
- Arcturus RiboAmp RNA amplification from fly brains: File:RiboAmp1.docx
- Basic RT reaction using ABI Archive cDNA kit: File:RT protocol.docx
- RNA prep using Trizol and Qiagen RNeasy columns: File:TrizolRNAProtocol.v1.pdf
- Protein Extract RIPA Recipe from Xiping Zhang at Esser Lab: File:EsserRIPABuffer.pdf
- New cDNA reverse transcription protocol for WUSTL: File:CDNAprotocol.wustl.pdf
- Human Muscle RNA Extraction from L.R. File:RNA extraction from human muscle.pdf
Cell Culture
- C2C12 Harvest for Protein Lysate: File:Cultured C2C12s to Protein Lysate.pdf
Protein Biology
- Tentative Western Protocol: File:WesternProtocolHughes31July2019.pdf
Mouse Colony Management, Genotyping, etc.
- Mouse tail digestion protocol for genotyping: File:MouseTailingProtocol.pdf
- PCR and Genotyping Protocol: File:HughesGenotypingProtocol.pdf
- EtBr Gel Imaging Settings: File:EtBrGelImagingSettings.pdf
Next-generation sequencing
- Pilot protocol for PCR amplifying fly RNA editing sites: File:Editingseq pilot.docx
- miRNA TruSeq Library Prep: File:MiRNA library prep protocol Illumina.pdf
Fly Behavior and Genetics
- Drosophila virgin collection: File:Drosophila virgin collection.docx
- Vials for DAM analysis: File:Fly food for DAM analysis.docx
- Fly food protocol: File:Fly food.docx
Analytics
- Human temperature record analysis: File:Human Temp Analysis.docx
- RUM (RNA-Seq Unified Mapper) guide: File:RUM guide.pdf
- Basic linux commands: File:Linux commands.pdf
- Willie's Aligning Sheet (26 March 2019): File:ShellBashLinuxAligningSTAR.tips.pdf
Hughes Lab
Pulmonary and Critical Care Medicine
Washington University School of Medicine
