HughesLab:Protocols
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* Pilot protocol for PCR amplifying fly RNA editing sites: [[Image:editingseq_pilot.docx]]<br> | * Pilot protocol for PCR amplifying fly RNA editing sites: [[Image:editingseq_pilot.docx]]<br> | ||
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== Fly Behavior and Genetics == | == Fly Behavior and Genetics == | ||
* ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]] | * ''Drosophila'' virgin collection: [[Image:Drosophila_virgin_collection.docx]] | ||
| - | * Vials for DAM analysis: | + | * Vials for DAM analysis: [[Image:Fly_food_for_DAM_analysis.docx]] |
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== Analytics == | == Analytics == | ||
| - | * Human temperature record analysis: | + | * Human temperature record analysis: [[Image:Human_Temp_Analysis.docx]] |
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Current revision
Home Lab Members Research JTK_Cycle Publications Protocols Contact
Contents |
Molecular Biology
- Basic qPCR protocol using the ABI7900HT machine and Taqman probes: Image:QPCR protocol.docx
- Arcturus RiboAmp RNA amplification from fly brains: Image:RiboAmp1.docx
- Basic RT reaction using ABI Archive cDNA kit: Image:RT protocol.docx
- RNA prep using Trizol and Qiagen RNeasy columns: Image:RNA prep using Trizol.docx
Next-generation sequencing
- Pilot protocol for PCR amplifying fly RNA editing sites: Image:Editingseq pilot.docx
Fly Behavior and Genetics
- Drosophila virgin collection: Image:Drosophila virgin collection.docx
- Vials for DAM analysis: Image:Fly food for DAM analysis.docx
Analytics
- Human temperature record analysis: Image:Human Temp Analysis.docx
Hughes Lab
Department of Biology
University of Missouri, St. Louis
(Starting in August, 2013)
