Huang: Agarose Slides for Imaging - Revision history

Carolina Tropini: /* Materials */

Carolina Tropini — Sun, 08 Mar 2009 22:52:51 GMT

Materials

←Older revision		Revision as of 22:52, 8 March 2009
Line 5:		Line 5:
	==Materials==		==Materials==

-	*~~500μg~~ Agarose	+	*500mg Agarose
	*100 ml PBS buffer/bacterial media		*100 ml PBS buffer/bacterial media
	*2 Glass microscope slides		*2 Glass microscope slides

Carolina Tropini: /* Procedure */

Carolina Tropini — Sat, 21 Feb 2009 20:04:15 GMT

Procedure

←Older revision		Revision as of 20:04, 21 February 2009
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	##Leave to dry for a couple of minutes		##Leave to dry for a couple of minutes
	#Add a cover slip on top, seal with nail polish at all 4 corners.		#Add a cover slip on top, seal with nail polish at all 4 corners.
		+	#You are now ready to image some cells!

	==Notes==		==Notes==

Carolina Tropini: /* Contact */

Carolina Tropini — Sat, 21 Feb 2009 19:58:14 GMT

Contact

←Older revision		Revision as of 19:58, 21 February 2009
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	==Contact==		==Contact==
-	*[[~~OpenWetWare~~:Carolina Tropini]]	+	*[[User:Carolina Tropini\|Carolina Tropini]]

Carolina Tropini: /* Contact */

Carolina Tropini — Sat, 21 Feb 2009 19:55:30 GMT

Contact

←Older revision		Revision as of 19:55, 21 February 2009
Line 30:		Line 30:

	==Contact==		==Contact==
-	*[[OpenWetWare:~~User\|~~Carolina Tropini]]	+	*[[OpenWetWare:Carolina Tropini]]

Carolina Tropini: /* Contact */

Carolina Tropini — Sat, 21 Feb 2009 19:55:08 GMT

Contact

←Older revision		Revision as of 19:55, 21 February 2009
Line 30:		Line 30:

	==Contact==		==Contact==
-	*[[Carolina Tropini]]	+	*[[OpenWetWare:User\|Carolina Tropini]]

Carolina Tropini: New page: ==Overview== This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top. ==Materials== 500μg Agarose 100 ml PBS buffer...

Carolina Tropini — Sat, 21 Feb 2009 19:53:03 GMT

New page: ==Overview== This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top. ==Materials== *500μg Agarose *100 ml PBS buffer...

New page

==Overview==

This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top.

==Materials==

*500μg Agarose
*100 ml PBS buffer/bacterial media
*2 Glass microscope slides
*4 Glass cover slips
*Nail Polish
*Bacterial liquid culture (at least 10 μl, ideally in exponential phase)

==Procedure==
#Mix Agarose with buffer solution, microwave until boiling.
#Prepare one glass slide and position 2 thin cover slips on each end, to create 2 edges.
#Pipet 25 μl of the Agarose solution onto the middle of the glass slide, while making sure not to touch the coverslips.
##Quickly position the second glass slide on top of the molten gel drop.
##Leave to dry for a few minutes.
#Remove the top glass slide.
# Add 2-4 μl of the liquid culture onto the hardened gel drop.
##Leave to dry for a couple of minutes
#Add a cover slip on top, seal with nail polish at all 4 corners.

==Notes==
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

==Contact==
*[[Carolina Tropini]]


[[Category:Protocol]]

[[Category:Escherichia coli]]

Huang: Agarose Slides for Imaging - Revision history

Carolina Tropini: /* Materials */

Carolina Tropini: /* Procedure */

Carolina Tropini: /* Contact */

Carolina Tropini: /* Contact */

Carolina Tropini: /* Contact */

Carolina Tropini: New page: ==Overview== This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top. ==Materials== *500μg Agarose *100 ml PBS buffer...

Carolina Tropini: New page: ==Overview== This protocol allows live bacterial cell imaging. We create a small, nutrient rich gel pad and inject the bacteria on top. ==Materials== 500μg Agarose 100 ml PBS buffer...