Hop DNA Isolation
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(→Hop DNA Extraction Protocol)
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Buffer: : tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM. Then add 200mg/of CTAB and /2-mercaptoethanol.
Revision as of 16:38, 5 March 2007
Hop DNA Extraction Protocol
- Obtain an adequate amount of fresh hop leaves and crush them with liquid Nitrogen and a small amount of Carborundum powder (fine 320 grit).
- Assume 90% of mass is water weight.
Buffer: 100ml: tris/HCl (ph=8.0) 50mM, NaCl 1.8M, EDTA 50mM. Then add 200mg/ml of CTAB and 20 μl/ml 2-mercaptoethanol.