Hoatlin:Reagent Requests

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==Additional Antibodies to Consider==
==Additional Antibodies to Consider==
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Other antibodies are available from the [http://www.ohsu.edu/genetics/fa/index.cfm| OHSU Fanconi Antibody Project].  These antibodies were made against peptides and were then affinity purified. Stacie and Alexis have tested most of them against the epitope-tagged, overexpressed target proteins. For results of our antibody tests, click [[Media:MonnatABs.pdf|here]]. In summary, we like antibodies for:
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There are other antibodies available from the FA cell repository at OHSU (in partnership with the Fanconi Anemia Research Fund) which are distributed free of charge to FA researchers [[http://www.ohsu.edu/genetics/fa/index.cfm| OHSU Fanconi Antibody Project]].  These antibodies were made against peptides and are affinity purified. Our lab has tested most of them against the epitope-tagged, overexpressed target proteins. For results of our antibody tests, click [[Media:MonnatABs.pdf|here]]. In summary, we like antibodies for:
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*FANCC, #2 (#1 has some ugly non-specific bands)
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*FANCC, #2 (#1 has too many non-specific bands)
*FANCD2, both
*FANCD2, both
*FANCF
*FANCF
*FANCG, #2
*FANCG, #2
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FANCG #1 and FANCE antibodies did not work for us.  
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The FANCG #1 and FANCE antibodies did not work for us.  
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Testing of FANCJ and USP antibodies will commence soon. Here is an account of the progress:
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The repository now has FANCJ and USP-1 antibodies availble!! Testing of these antibodies by our lab will commence soon. Here is an account of the progress:
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*Early June, FANCJ/USP Abs requested
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*Early June, FANCJ/USP Abs requested from the repository
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*June 16, Rec'd confirmation of request
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*June 16, Rec'd confirmation of request, sent in MTA
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*July 27...still waiting
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*July 27...still waiting for arrival
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*Aug 28....still waiting
Back to [[Hoatlin Lab]]
Back to [[Hoatlin Lab]]

Revision as of 02:51, 29 August 2006

Hoatlin Antibodies

We are committed to creating and maintaining top-quality, characterized antibodies for Fanconi research. We have shipped our antibodies to approximately 55 labs in 11 different countries. To request characterized antibodies or other published reagents from us, please contact Alexis at lachapel_AT_ohsu_DOT_edu. For antibodies, please include enough detail so we can send you the best antibody, estimate how much you'll need, and send us your FedEx number and shipping information. For bookkeeping purposes kindly fill out this MTA and FAX or email it back to Alexis (lachapel_AT_ohsu_DOT_edu). Our fax number is 503-494-8393.

The links below will lead you to a pdf listing some of the antibodies we have available. We are running very low on some of the good ones, so we ask you to estimate how much you need. You can always ask for more if it works! Citations relating to the antibodies are listed in the "Antibody References" link. See our secret Protocols link for getting the antibodies to work. Feel free to contact us with any questions. If you don't see what you need, let us know. Thanks to the Fanconi Anemia Research Fund for supporting this effort and to our collaborators in the labs of Hans Joenje and Weidong Wang for working together so effectively.

Additional Antibodies to Consider

There are other antibodies available from the FA cell repository at OHSU (in partnership with the Fanconi Anemia Research Fund) which are distributed free of charge to FA researchers [OHSU Fanconi Antibody Project]. These antibodies were made against peptides and are affinity purified. Our lab has tested most of them against the epitope-tagged, overexpressed target proteins. For results of our antibody tests, click here. In summary, we like antibodies for:

  • FANCC, #2 (#1 has too many non-specific bands)
  • FANCD2, both
  • FANCF
  • FANCG, #2

The FANCG #1 and FANCE antibodies did not work for us.

The repository now has FANCJ and USP-1 antibodies availble!! Testing of these antibodies by our lab will commence soon. Here is an account of the progress:

  • Early June, FANCJ/USP Abs requested from the repository
  • June 16, Rec'd confirmation of request, sent in MTA
  • July 27...still waiting for arrival
  • Aug 28....still waiting

Back to Hoatlin Lab

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