Hoatlin:Reagent Requests

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{{HoatlinLab}}
==Hoatlin Antibodies==
==Hoatlin Antibodies==
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We are committed to creating and maintaining top-quality, characterized antibodies for Fanconi research.  We have shipped our antibodies to approximately [[Hoatlin:Labs we've shipped to:|55 labs in 11 different countries]].  To request characterized antibodies or other published reagents from us, please contact Alexis at lachapel_AT_ohsu_DOT_edu.  For antibodies, please include enough detail so we can send you the best antibody, estimate how much you'll need, and send us your FedEx number and shipping information. For bookkeeping purposes kindly fill out this [[Media:MTA.pdf|MTA]] and FAX or email it back to Alexis. Our fax number is 503-494-8393.
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We are committed to creating and maintaining top-quality, characterized antibodies for Fanconi research.  We have shipped our antibodies to approximately [[Hoatlin:Labs we've shipped to:|65 labs in 12 different countries]].  To request characterized antibodies or other published reagents from us, please contact Maureen at hoatlinm AT ohsu DOT edu.  For antibodies, please include enough detail so we can send you the best antibody, estimate how much you'll need, and send us your FedEx number and shipping information. Kindly fill out this [[Media:HoatlinMTA07.pdf|MTA]] and follow the instructions. Please note that the university requires the MTA to be completed before our lab can be authorized to send the material.
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The links below will lead you to a pdf listing some of the antibodies we have available. We are running very low on some of the good ones, so we ask you to estimate how much you need. You can always ask for more if it works!  Citations relating to the antibodies are listed in the "Antibody References" link. See our secret Protocols link for getting the antibodies to work. Feel free to contact us with any questions. If you don't see what you need, let us know. Thanks to the Fanconi Anemai Research Fund for supporting this effort and to our collaborators in the labs of Hans Joenje and [http://www.grc.nia.nih.gov/branches/irp/wwang.htm Weidong Wang] for working together so effectively.
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The links below will lead you to a pdf listing some of the antibodies we have available. We are running very low on some of the good ones, so we ask you to estimate how much you need. You can always ask for more if it works!  Citations relating to the antibodies are listed in the "Antibody References" link. See our secret Protocols link for getting the antibodies to work. Feel free to contact us with any questions. If you don't see what you need, let us know. Thanks to the [http://www.fanconi.org/research/Supported.htm Fanconi Anemia Research Fund] for supporting this effort and to our collaborators in the labs of Hans Joenje and [http://www.grc.nia.nih.gov/branches/irp/wwang.htm Weidong Wang] for working together so effectively.
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[[Media:FANCAntibodies.pdf|Antibodies directed against FA proteins]]
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*Many of our lab's Fanconi Anemia antibodies are available from [http://www.novusbio.com Novus Biologicals]
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*[[Hoatlin:Antibody Data sheets|Antibody Data sheets]]
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[[Hoatlin Lab:Antibody references|Antibody References]]
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*[[Media:FANCAntibodies.pdf|Antibodies directed against FA proteins]]
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[[Hoatlin:Protocols| Protocols]]
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*[[Hoatlin Lab:Antibody references|Antibody References]]
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*[[Hoatlin:Protocols| Protocols]]
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*[[Help:Contents | Edit help for this section or to make your own lab wiki]]
==Additional Antibodies to Consider==
==Additional Antibodies to Consider==
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Other antibodies are available from the [http://www.ohsu.edu/genetics/fa/index.cfm| OHSU Fanconi Antibody Project].  These antibodies were made against peptides and were then affinity purified. Stacie and Alexis have tested most of them against the epitope-tagged, overexpressed target proteins. For results of our antibody tests, click [[Media:MonnatABs.pdf|here]]. In summary, we like antibodies for:
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There are other antibodies available from the FA cell repository  of the Fanconi Anemia Research Fund which are distributed free of charge to FA researchers from the [http://www.fanconi.org/research/Materials.htm#Antibodies| Fanconi Antibody Project].  These antibodies were made against peptides and are affinity purified. Our lab has tested most of them against the epitope-tagged, overexpressed target proteins. '''For results of our antibody tests, click [[Media:MonnatABs.pdf|here]].''' In summary, we like antibodies for:
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*FANCC, #2 (#1 has some ugly non-specific bands)
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*FANCC, #2 (#1 has too many non-specific bands)
*FANCD2, both
*FANCD2, both
*FANCF
*FANCF
*FANCG, #2
*FANCG, #2
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FANCG #1 and FANCE antibodies did not work for us.  
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The FANCG #1 and FANCE antibodies did not work for us.  
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Testing of FANCJ and USP antibodies will commence soon.
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Johan deWinter's laboratory has tested the antibodies independently for endogenous detection of the FA proteins in lymphoblasts. Johan's results are summarized below and the raw data with blots can be downloaded [[Media:hoatlinFARFresultsjdeW.pdf|here]].
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== Results: FARF Antibodies tested on overexpressed and endogenous targets==
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<div style="padding: 10px; width: 720px; border: 5px solid #B3CD4E;">
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{| border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse; width:710px" <!-- This line here formats your table for you.  Change the code to change the formatting of your table.-->
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| align="center" style="background:#f0f0f0;"|'''Antibody'''
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| align="center" style="background:#f0f0f0;"|'''Direct Western Blot'''
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| align="center" style="background:#f0f0f0;"|'''IP>WB'''
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| align="center" style="background:#f0f0f0;"|'''Comments'''
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|--
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| FANCC (1)
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| Not visible
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| Not visible   
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| Hoatlin&deWinter results agree
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|--
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|FANCC (2)
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|Visible (1:5000)[[Media:hoatlinFARFresultsjdeW.pdf|See picture #1]]
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|Not visible
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|Hoatlin&deWinter results agree, direct western has very clear band 
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|--
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|FANCD1 (1)
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|Visible (1:5000)[[Media:hoatlinFARFresultsjdeW.pdf|See picture #4]]
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|Not tested
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|very clear band on direct western
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|--
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|FANCD2 (2)
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|Visible (1:5000)[[Media:hoatlinFARFresultsjdeW.pdf|See picture #5]]
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|Not tested
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|Hoatlin&deWinter results agree, very clear double band on direct western
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|--
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|FANCE
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|Visible (1:5000)[[Media:hoatlinFARFresultsjdeW.pdf|See picture #2]]
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|Not visible?
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|after 10 min with Super ECL
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|--
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|FANCF
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|Not visible
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|Not visible
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|Hoatlin&deWinter results agree
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|--
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|FANCG (1)
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|Not visible
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|Not visible
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|Hoatlin&deWinter results agree
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|--
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|FANCG (2)
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|Not visible
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|Visible (1:500). IP Ab GP12 [[Media:hoatlinFARFresultsjdeW.pdf|See picture #3]]
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|Hoatlin&deWinter results agree
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|--
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|}
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</div>
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The repository now has FANCJ, USP-1 FANCA, FANCB, FANCM and FANCN available.
Back to [[Hoatlin Lab]]
Back to [[Hoatlin Lab]]

Current revision

Equipped with his five senses, man explores the universe around him and calls the adventure Science.  ~Edwin Powell Hubble, The Nature of Science, 1954

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Hoatlin Antibodies

We are committed to creating and maintaining top-quality, characterized antibodies for Fanconi research. We have shipped our antibodies to approximately 65 labs in 12 different countries. To request characterized antibodies or other published reagents from us, please contact Maureen at hoatlinm AT ohsu DOT edu. For antibodies, please include enough detail so we can send you the best antibody, estimate how much you'll need, and send us your FedEx number and shipping information. Kindly fill out this MTA and follow the instructions. Please note that the university requires the MTA to be completed before our lab can be authorized to send the material.

The links below will lead you to a pdf listing some of the antibodies we have available. We are running very low on some of the good ones, so we ask you to estimate how much you need. You can always ask for more if it works! Citations relating to the antibodies are listed in the "Antibody References" link. See our secret Protocols link for getting the antibodies to work. Feel free to contact us with any questions. If you don't see what you need, let us know. Thanks to the Fanconi Anemia Research Fund for supporting this effort and to our collaborators in the labs of Hans Joenje and Weidong Wang for working together so effectively.

Additional Antibodies to Consider

There are other antibodies available from the FA cell repository of the Fanconi Anemia Research Fund which are distributed free of charge to FA researchers from the Fanconi Antibody Project. These antibodies were made against peptides and are affinity purified. Our lab has tested most of them against the epitope-tagged, overexpressed target proteins. For results of our antibody tests, click here. In summary, we like antibodies for:

  • FANCC, #2 (#1 has too many non-specific bands)
  • FANCD2, both
  • FANCF
  • FANCG, #2

The FANCG #1 and FANCE antibodies did not work for us.

Johan deWinter's laboratory has tested the antibodies independently for endogenous detection of the FA proteins in lymphoblasts. Johan's results are summarized below and the raw data with blots can be downloaded here.

Results: FARF Antibodies tested on overexpressed and endogenous targets

Antibody Direct Western Blot IP>WB Comments
FANCC (1) Not visible Not visible Hoatlin&deWinter results agree
FANCC (2) Visible (1:5000)See picture #1 Not visible Hoatlin&deWinter results agree, direct western has very clear band
FANCD1 (1) Visible (1:5000)See picture #4 Not tested very clear band on direct western
FANCD2 (2) Visible (1:5000)See picture #5 Not tested Hoatlin&deWinter results agree, very clear double band on direct western
FANCE Visible (1:5000)See picture #2 Not visible? after 10 min with Super ECL
FANCF Not visible Not visible Hoatlin&deWinter results agree
FANCG (1) Not visible Not visible Hoatlin&deWinter results agree
FANCG (2) Not visible Visible (1:500). IP Ab GP12 See picture #3 Hoatlin&deWinter results agree


The repository now has FANCJ, USP-1 FANCA, FANCB, FANCM and FANCN available.

Back to Hoatlin Lab

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