HighPoint/CannonLab:Buffer and Osmolyte Solutions: Difference between revisions
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(New page: {{CannonLabMenu}} ==Preparing Buffers== ===Stock Solutions=== # Prepare stock solutions of buffer components (typically 1 M) # Rinse ''clean'' storage bottle and filter flask an ''extra...) |
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Revision as of 09:32, 16 February 2012
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Preparing Buffers
Stock Solutions
- Prepare stock solutions of buffer components (typically 1 M)
- Rinse clean storage bottle and filter flask an extra time
- Use type I, 18 MΩ purified water
- Drain and use your best aseptic technique from this point on
- Filter stock solutions through 0.2 μm filter
- Store in bottle for future use
- Keep the bottle closed as much as possible
- Always wear gloves and use clean instruments for removing aliquots
- This will extend the life of your stock solution before bacteria begin to grow in it